Genox

'High Sensitive 8-OHdG Check’  ELISA Kit
(ELISA kit for 8-hydroxy-2’-deoxyguanosine)
Catalog #KOG-HS10E

Product Insert (pdf)


High Sensitive 8-OHdG Check is a competitive in vitro enzyme-linked immuno-sorbent assay for the quantitative measurement of the oxidative DNA adduct, 8-hydroxy-2'-deoxyguanosine (8-OHdG) in test samples such as DNA of tissues, lymphocytes, cell culture medium etc., that are expected to have low levels of 8-OHdG.

Sample Pre-Treatment Protocol (pdf)
Please note in most cases sample pre-treatment is required to measure the free form of 8-OHdG.

 

Differences between the 'New 8-OHdG Check'
and High Sensitive 8-OHdG Check ELISA Kit

 

New 8-OHdG
Check ELISA

High Sensitive 8-OHdG
Check ELISA

Measurement Range

0.5-200 ng/ml

0.125-10 ng/ml

Primary Antibody Reaction

37°C, 1hour

4°C, over night

Secondary Antibody Reaction

37°C, 1hour

room temperature, 1hour

 

Features of the High Sensitive 8-OHdG Check ELISA Kit

1.

Easy operation:

There is no need for expensive equipments and sample pretreatment.

2.

Highly specific:

Monoclonal antibody recognizes 8-OHdG specifically
(21 analogues of 8-OHdG do not have any cross reactivity).

3.

Speed:

Estimated time of assay is overnight incubation followed
by 2 hours.

4.

With blank and standard 24 samples can be tested in triplicate. Only 150µl
of sample is required (50µl per well).

5.

The use of split type microplate prevents the waste of reagents.


Merits of the High Sensitive 8-OhdG Check Elisa Kit

The High Sensitive 8-OHdG Check is useful as a non-invasive indicator in numerous ways, such as:

  • Use as a screening protocol to learn the role of oxidative stress biomarker 8-OHdG in aging and for application of this biomarker in the advancement of basic and applied research in biology and medicine.
  • In nutrition, diet, and life style related basic and applied research.
  • In evaluating the extent of oxidative stress generated as a result of exercises performed by the body or specific actions to which the body is subjected to.
  • In determining the side effects of oxidative stress following clinical procedures
    or intake of  therapeutic drugs.


Kit Contents

  • 8-OHdG pre-coated microtiter plate (split type)
  • Primary antibody (Anti-8-OHdG monoclonal antibody)
  • Primary antibody reconstituting buffer
  • Secondary antibody (HRP-conjugated anti mouse antibody)
  • Secondary antibody reconstituting buffer
  • Chromatic solution (3,3',5,5'-tetramethylbenzidine)
  • Diluting solution
  • Washing solution
  • Reaction terminating solution
  • Standard 8-OHdG solution (0.125, 0.25, 0.5, 1, 4, 10 ng/ml)
  • Plate seal


Assay Procedure

(1)  Primary Antibody Reaction (Competitive Reaction): 4°C for 1hour
(2)  Secondary Antibody Reaction: Room temperature for 1hour
(3)  Development of Color Reaction: Room Temperature for 15min in the dark
(4)  Absorbance Reading (wavelength at 450 nm) and Calculation of Results


REFERENCES

  1. S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi, and T.Osawa:
    Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model.
    Lab. Invest. 76(3), p365-374 (1997)
    Information about specificity of anti 8-OHdG monoclonal antibody.

  2. Suzuki K, et. al.:
    The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents.
    J Epidemiol 13(1):29-37 (2003)
    Application to normal human serum.

  3. Watanabe E, Matsuda N, Shiga T, Kajimoto K, Ajiro Y, Kawarai H, Kasanuki H, Kawana M:
    Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy.
    J Card Fail. 12(7):527-32(2006)
    Serum 8-OHdG in patients.

  4. Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K:
    Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage.
    J Neurol Sci. 250(1-2):159-61(2006)
    8-OHdG in CSF, plasma and urine samples.

  5. M Takane, et. al.
    New biomarker evidence of oxidative DNA damage in whole saliva from clinically healthy and periodontally diseased individuals.
    J Periodontol. 73(5):551-4.(2002)
    8-OHdG in human saliva samples are assessed.

  6. M Kakimoto, T Inoguchi, T Sonta, HY Yu, M Imamura, T Etoh, T Hashimoto and H Nawata:
    Accumulation of 8-hydroxy-2'-deoxyguanosine and mitochondrial DNA deletion in kidney of diabetic rats.
    Diabetes 51, p1588-1595(2002)
    8-OHdG in tissue nuclear and mitochondrial DNA are detected.

 

The 'High Sensitive 8-OHdG Check ELISA Kit' is manufactured in Japan by the Japan Institute for the Control of Aging (JaICA). Genox is the distributor of this kit for users in North and South America.

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Note: Genox’s Products are for RESEARCH USE ONLY.
Users of Genox’s products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC APPLICATIONS. 

The provision of test reports, generated by using Genox's products, to individuals
or to treating physicians for the diagnosis, prevention, treatment and control of any human disease or impairment of, or the assessment of the health, nutritional, or medical condition of individuals is expressly prohibited by law.  42 U.S.C. § 263a (2006); 42 C.F.R. § 493.3 (2006); COMAR 10.10.01.02 (2006). Genox is not a CLIA certified laboratory.

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