Genox

Hexanoyl-Lysine  ELISA
(ELISA kit for 13-HPODE Lysine adduct)
Catalog #KHL-700E

Product Insert (pdf)


What is HEXANOYL-LYSINE?

Oxidative damage of lipids caused by reactive oxygen species (ROS) plays an important role in the lesion of cell functions and aging. Aldehydes such as malondialdehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as the advanced lipid peroxidation products. In the earlier stages of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct  is a novel lipid hydroperoxide-modified lysine residue. It  is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid.  Using this kit, Hexanoyl-Lysine can be measured in human urine and serum.

Specifications

Assay principle:

Competitive ELISA (detection: 450 nm)

Specificity:

Specific to Hexanoyl-Lysine

Measuring range:

2 - 700 nmol/L

Time Required:

Over night and 2 hours

Format:

96 wells microplate

Applications:

Urine and serum samples from human and animals.

Storage:

Store at 2 - 8°C 

Expiry:

12 months from the  manufacturing date

Required but not provided:

50 micro L micropipette and pipette tips
8-channel (50-200 micro L) micropipette and pipette tips
8 or 12-synchronous multi-channel pipette and reagent tray for multi-channel pipette.
4 - 7 degree C incubator Microtiter plate reader (measuring wavelength 450 nm)

 

Content of this kit

 

 

HEL-coated Microtiter Plate:

1 plate (96 wells)

Primary Antibody (ready to use):

1 vial

Secondary Antibody:

1 vial

Secondary Antibody Buffer:

1 vial

Chromogen (TMBZ solution):

1 vial

Chromogen Buffer:

1 vial

Washing Buffer (5X):

1 vial

Stop Solution:

1 vial

Standard solution (6 levels):

1 vial each

Plate seal:

2 sheets


HEL ELISA Kit Assay Procedure


REFERENCES

  1. Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide.
    Y. Kato, Y. Mori, Y. Makino, Y. Morimitsu, S. Hiroi, T. Ishikawa, T. Osawa.
    J. Biol. Chem. 274(29), p20406-20414 (1999)

  2. Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies.
    Y. Kato, T. Osawa
    Methods in Molecular Biology, vol. 186, Oxidative stress biomarkers and antioxidant protocols, D Armstrong, Ed., Human Press Inc., NJ, USA, p37-44

  3. Preparation of a monoclonal antibody to N epsilon-(hexanonyl) lysine: applycation to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle.
    Y. Kato, Y. Miyake, K. Yamamoto, Y. Shimomura, H. Ochi, Y. Mori, T. Osawa
    Biochem. Biophys. Res. Commun. 274(2), p389-393 (2000)

  4. The protective effects of tetrahydrocurcumin on oxidative stress in cholesterol-fed rabbits.
    M. Naito, Wu X, H. Nomura, M. Kodama, Y. Kato, Y. Kato, T. Osawa
    J Atheroscler Thromb. 9(5), p243-250 (2002)

  5. Formation of Nepsilon-(hexanonyl)lysine in oxidized human very-low density lipoprotein.
    H. Arai, Y. Kato, K. Fukunaga, S. Mohri, and K. Nakamura
    J. Electrophoresis 48, p37-40 (2004)

  6. Identification and Quantification of N-epsilon-(Hexanoyl)lysine in human urine by liquid chromatography/tandem mass spectrometry.
    Y. Kato, A. Yoshida, M. Naito, Y. Kawai, K. Tsuji, M. Kitamura, N. Kitamoto, and T. Osawa
    Free Radic. Biol. Med. 37(11), p1864-1874 (2004)

 

The ‘Hexanoyl-Lysine ELISA Kit’ is manufactured in Japan by the Japan Institute for the Control of Aging (JaICA). Genox is the distributor of this kit for users in North and South America.

ORDER THIS PRODUCT

Note: Genox’s Products are for RESEARCH USE ONLY.
Users of Genox’s products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC APPLICATIONS. 

The provision of test reports, generated by using Genox's products, to individuals
or to treating physicians for the diagnosis, prevention, treatment and control of any human disease or impairment of, or the assessment of the health, nutritional, or medical condition of individuals is expressly prohibited by law.  42 U.S.C. § 263a (2006); 42 C.F.R. § 493.3 (2006); COMAR 10.10.01.02 (2006). Genox is not a CLIA certified laboratory.

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