Genox

Anti-Crotonaldehyde Monoclonal Antibody
(Anti CRA monoclonal antibody) 
(Catalog #MCRA-030 for 30 microgram vial)

Product Insert (pdf)


What is Crtotonaldehyde?

Crotonaldehyde (CRA) is a representative carcinogenic aldehyde formed endogenously through lipid peroxidation. CRA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with CRA and lysine residue leads to the formation of numerous numbers of adducts. This antibody is specific for the CRA-modified protein.

Subclass

:

Mouse IgG2a

Antigen

:

CRA-modified keyhole-lympet hemocyanine

Specificity

:

Specific for CRA-modified protein
(especially CRA-lysine adduct)

Form

:

Frozen (100 micro g/mL antibody in 10mM PBS containing
0.1%NaN3 and 0.5% BSA). Purified by Protein-A.

Storage

:

Less than -20°C

Application

:

Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 micro gram/mL on paraformaldehyde fixed tissue.

 

IMMUNOHISTOCHEMISTRY
1)

Paraffin sections were deparaffinized and rehydrated.
2)

Sections were quenched for several minutes with 3% hydrogen peroxide, rinsed in PBS, pretreated for 30 min with 3% nonimmune animal serum in PBS.
3)

Sections were incubated overnight at 4°C with a antibody at a concentration of 0.5-10 micro gram/mL.
4)

Antibody binding was visualized by the avidin-biotin-immuno peroxidase complex method using the Vectastain ABC kit (Vector, Burlingame, CA) according to the manufacturer's instructions.
5)

3,3-Diaminobenzidine tetrahydrochloride was used as the chromogen.
6)

Hematoxylin or Eosin was used as the counter stain.

 

Technical Note
1)

Sections from which the primary antibodies were omitted served as negative reaction controls.
2)

For frozen materials, samples were fixed in 10% formalin, immersed in 30% sucrose in PBS, embedded at OCT (Sakura, Tokyo, Japan), and stored at 80°C.
3)

For paraffin embedded materials, samples were fixed in 10% formalin, dehydrated, embedded in paraffin, and stored at room temperature.
4)

Microwave treatment improves the target staining, whereas it declines
non-specific background.
5)

Protease K treatment declines the target staining.

Positive control
Aortic wall in the renal tissue of diabetic nephropathy or aortic wall of atherosclerosis.

The ‘Anti-CRA Monoclonal Antibody’ is manufactured in Japan
by the Life Science Products Division of the Nippon Oils and Fats
Corporation, Japan. Genox is the distributor of this product for users
in North and South America.

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Note: Genox’s Products are for RESEARCH USE ONLY.
Users of Genox’s products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC APPLICATIONS. 

The provision of test reports, generated by using Genox's products, to individuals
or to treating physicians for the diagnosis, prevention, treatment and control of any human disease or impairment of, or the assessment of the health, nutritional, or medical condition of individuals is expressly prohibited by law.  42 U.S.C. § 263a (2006); 42 C.F.R. § 493.3 (2006); COMAR 10.10.01.02 (2006). Genox is not a CLIA certified laboratory.

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