Anti-Malondialdehyde Monoclonal Antibody
(Anti
Malondialdehyde monoclonal antibody, clone #1F83)
(Catalog #MMDA-030 for 30 microgram vial)
What is Malondialdehyde?
Malondialdehyde (MDA) is one of the major aldehydes derive from
lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine
residue in protein. The reaction with MDA and lysine residue leads to
the formation of numerous numbers of adducts, such as dihydropyridine-lysine
(DHP-lysine) type derivative. This monoclonal antibody is specific for
the MDA-modified protein, especially DHP-lysine type derivative.
Clone # |
: |
1F83 |
Antigen |
: |
MDA-modified keyhole-lympet hemocyanine. |
Form |
: |
Frozen (100 micro g/mL antibody in 10mM PBS containing 0.1 %NaN3 and 0.5% BSA). Purified by Protein-A. |
Application |
: |
Immunohistochemistry. |
Specificity |
: |
Specific for MDA-modified protein (especially DHP-lysine). |
Subclass |
: |
Mouse IgG2a(lambda) |
Storage |
: |
Less than -20°C |
Specificity of anti-MDA antibody.
Immunohistochemical detection of MDA-modified protein in atherosclerotic
aorta.
Dr. N Shibata, Tokyo Women's Medical University.
IMMUNOHISTOCHEMISTRY Procedure |
|
1) |
Paraffin sections were deparaffinized and rehydrated. |
2) |
Sections were quenched for several minutes with 3% hydrogen peroxide, rinsed in PBS, pretreated for 30 min with 3% nonimmune animal serum in PBS. |
3) |
Antigen retrieval by microwave treatment. |
4) |
Sections were incubated overnight at 4°C with a antibody at a concentration of 0.5-10 micro gram/mL. |
5) |
Antibody binding was visualized by the avidin-biotin-immuno peroxidase complex method using the Vectastain ABC kit (Vector, Burlingame, CA) according to the manufacturer's instructions. |
6) |
3,3-Diaminobenzidine tetrahydrochloride was used as the chromogen. |
7) |
Hematoxylin or Eosin was used as the counter stain.
|
REFERENCES
1) |
S Yamada, et. al.: Immunochemical detection
of a lipofuscin-like fluophore derived from malondialdehyde and
lysine. |
The ‘Anti-MDA Monoclonal Antibody’ is
manufactured in Japan
by the Life Science Products Division of the
Nippon Oils
and Fats
Corporation, Japan. Genox is the distributor of this product
for users in North and South
America.
Note: Genox’s Products are
for RESEARCH USE ONLY.
Users of Genox’s
products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC
APPLICATIONS.
The provision of test reports, generated
by using Genox's products, to individuals
or to
treating physicians for the diagnosis, prevention,
treatment and control of any human disease or impairment
of, or the assessment of the health, nutritional,
or medical condition of individuals is expressly
prohibited by law. 42
U.S.C. § 263a (2006); 42 C.F.R. § 493.3
(2006); COMAR 10.10.01.02 (2006). Genox
is not a CLIA certified laboratory.
© Copyright 1996-2008 Genox Corporation. All rights reserved.
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