Clone #

:

N45.1

Antigen

:

8-OHdG-conjugated Keyhole Limpet Hemocyanin

Subclass

:

Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified.

Form

:

Lyophilized Powder

Specificity

19 analogues of 8-OHdG {guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, Uric acid, Urea, creatine, creatinine} demonstrate no cross-reactivity. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%).

Use

Immunohistochemistry and ELISA

Storage

Less than -20°C for 5 years
When transport, stable at least 7 days at room temperature.

1)

S.Toyokuni, T.Tanaka, Y.Hattori, Y.Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi, and T.Osawa .   Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxy-guanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model.
Laboratory Investigation, 76(3), 365-374 (1997)

2)

T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion.
Lab. Invest. 77(2), p145-155 (1997)

3)

Y.Hattori, C.Nishigori, T.Tanaka, K.Uchida, O.Nikaido, T.Osawa, H.Hiai, S.Imamura, and S.Toyokuni: 8-Hydroxy-2'deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure.
J. Invest. Dermatol. 107, p733-737 (1997)

4)

S.Takahashi, M.Hirose, S.Tamano, M.Ozaki, S.Orita, M.Takeuchi, H.Ochi, S.Fukuda, H.Kasai, and T.Shirai: Immunohistochemical detection of 8-hydroxy-2’-deoxyguanosine in paraffin-embedded sections of rat liver after carbon tetrachloride treatment.
Toxicol. Pathol. 26, p247-252 (1998)

5)

S.Toyokuni.  Reactive oxygen species-induced molecular damage and its application in pathology.
Pathology International, 49, 91-102 (1999) 

6)

S.Toyokuni, Y.Iwasa, S.Kondo, T.Tanaka, H.Ochi, and H.Hiai.  Intranuclear distribution of 8-hydroxy-2'-deoxyguanosine: An immunocytochemical study.
The Journal of Histochemistry and Cytochemistry, 47(6), 833-835 (1999)  

7)

S.Kondo, S.Toyokuni, Y.Iwasa, T.Tanaka, H.Onodera, H.Hiai, and M.Imamura
Persistent oxidative stress in human colorectal carcinoma, but not in adenoma.
Free Radical Biology and Medicine, 21(3/4), 401-410 (1999)  

8)

Y.Ihara, S.Toyokuni, K.Uchida, H.Okada, T.Tanaka, H.Ikeda, H.Hiai, Y.Seino, and Y.Yamada.  Hyperglycemia causes oxidative stress in pancreatic  cells of GK rats, a model of type 2 diabetes.
Diabetes, 48, 927-932 (1999)

9)

N.U.Ahmed, M.Ueda, O.Nikaido, T.Osawa and M.Ichihashi: High levels of 8-hydroxy-2'deoxyguanosine appear in normal human epidermis after a singer dose of ultraviolet radiation.
Br. J. Dermatol. 140, p226-231 (1999)

10)

S.Toyokuni: An in vitro Feton reaction model and the application of immunohistochemistry to detect oxidative damage.
Models and Methods in Cell Signaling and Gene Expression: Applications to Oxidative Stress Research., Chapter four pp40-54 (2000)

11)

K.Yamagami, Y.Yamamoto, M.Kume, Y.Ishikawa, Y.Yamaoka, H.Hiai and S.Toyokuni:
Formation of 8-hydroxy-2'deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: Distinct localization of the two oxidatively modified products.  Antioxidants & Redox Signaling, 2(1), p127-136 (2000)

12)

D.Nakae, H.Akai, H.Kishida, O.Kusuoka, M.Tsutsumi, and Y.Konishi: Age and organ dependent spontaneous generation of nuclear 8-hydroxydeoxyguanosine in male Fischer 344 rats.
Lab. Invest. 80(2), p249-261 (2000)

13)

S.Liardet, C.Scaletta, R.Panizzon, P.Hohlfeld and L.L.Applegate:
Protection against pyrimidine dimmers, p53, and 8-hydroxy-2'deoxyguanosine expression in ultraviolet-irradiated human skin by sunscreens: difference between UVB + UVA and UVB alone sunscreens.
J. Investigative Dermatol. 117(6), p1437-1441 (2001)

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