 |
| Suitable for immunohistocheistry and western blotting. For research use only. |
| About 4-hydroxy-2-nonenal (4-HNE) |
 Structure of 4-hydroxy-2-nonenal
Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies,
and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are
formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of
omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body
is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of
lipid peroxidation.
|
|
High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous
epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999) |
|
 |
| Specifications |
| Clone #: |
HNEJ-2 |
| Immunogen: |
4-HNE-modified Keyhole Limpet Hemocyanin |
| Subclass: |
Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified. |
| Form: |
Lyophilized Powder (containing 20 µg or 100 µg of IgG) |
| Specificity: |
This antibody show almost negligible reactivity with proteins that were treated with other
aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde. |
| Applications: |
Immunohistochemistry and western blotting. |
| Storage: |
Less than -20°C for 5 years. When transport, stable at least 7 days at room temperature. |
|
|
| References |
| Immunohistochemistry: |
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney:
independence of tubular necrosis and sulfhydryl depletion.
Lab. Invest. 77(2), p145-155 (1997) |
| Western blotting: |
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995) |
|
|
| Product name |
Code |
Content |
| Anti 4-HNE monoclobal antibody |
MHN-020P |
20 µg of IgG/ vial |
| MHN-100P |
100 µg of IgG/ vial |
|
Made in Japan
|
|
|
|
|
| Contact technical support |
|
|
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would causedby such faults, we will be exempt from responsibility.
|
Copyright ©
Genox Corporation. All rights reserved.
|
)