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Trace elenets assay kits.
Traceelements Magnesium colorimetric assay kit Traceelements Magnesium colorimetric assay kit Traceelements Magnesium colorimetric assay kit
Easy to use. For cell lysates, serum and wide variety of biological samples.

Pre-preatment free!
Species independent!
No toxic substance!
Applicable to microplate reader!
[ Product Description ]
Magnesium is one of the most abundant minerals in the living cells, and plays important roles in many metabolic reactions such as polyphosphoric acid formation and photosynthesis in the chlorophyll.

[ Assay principle ]
Under alkaline condition, Xyridyl Blue-I(XB-I) - magnesium complex shows absorbance at 520 nm, and on the other hand, the absorbance by free XB-I at 600-680nm decreases.

[ Performance ]
1. Sensitivity:
2. Accuracy:
3. Intra assay variation:
4. Assay range:
O.D. at 2 mg/dL standard will be 0.05 - 0.1(660nm vs 700nm)
±15% of known concentration
C.V. is less than 5%
0.05 - 3.5 mg/dL
[ Specifications ]
Principle: XB-I method (Colorimetric assay at 520-660 vs 700 nm)
Measuring wavelength: 660nm (reference 700 nm) is recommended.
Measuring at 520 nm vs 700 nm shows higher sensitivity but may be susceptible to various factors.
Specifity: Magnesium (Mg)
Assay range: 0.05 - 3.5 mg/dL
Assay condition: 5 minutes at room temperature (25 - 37 °C)
Applications: Serum, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. This assay will be interfered by EDTA, hemoglobin, anticoagulants and high concentration of potassium.
Storage: 2 - 8°C
Expiry: 1 year after the day of manufacturing.
Required but not provided: Distilled water
96 well microtiter plate
Micropipettors and pipette tips
Microtiter plate reader (measuring wavelength 660/700 nm)
[ Contents of this kit ]
R-1 Chelate color (ready to use): 25 mL
Magnesium standard 2 mg/dL (ready to use): 0.5 mL
[ References: Assay examples ]
1) Akanuma G, Kobayashi A, Suzuki S, Kawamura F, Shiwa Y, Watanabe S, Yoshikawa H, Hanai R, Ishizuka M:
Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.
J Bacteriol 196(22),p3820-3830(2014) doi: 10.1128/JB.01896-14
2) Sakamoto A, Terui Y, Yamamoto T, Kasahara T, Nakamura M, Tomitori H, Yamamoto K, Ishihama A, Michael AJ, Igarashi K, Kashiwagi K:
Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor.
Int J Biochem Cell Biol 44(11),p1877-1886(2012). doi: 10.1016/j.biocel.2012.07.010
Product name Catalog# Assay range Size Price
MC Reagent Magnesium Assay Kit (Xyridil Blue-I) CMG-035 0.05 - 3.5 mg/dL 100 tests US$310

Made in Japan.

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